Resources minister Matthew Canavan has officially ruled out proceeding with Wallerberdina Station in the Flinders Ranges as the site for the National Radioactive Waste Management Facility (NRWMF).
The news comes after a community ballot in Hawker showed 52.46 per cent of voters were against the facility.
Mr Canavan said while the community ballot formed only part of the consultation process, he acknowledged the result meant there was not broad community support for the facility.
"While the community ballot was just one of many measures I am considering, I have said that achieving at least a majority level of support was a necessary condition to achieving broad community support," Minister Canavan said.
"This ballot does not demonstrate a sufficient level of support and I will no longer consider this site an option for the facility."
Three sites have been considered, with the remaining two options both in the Kimba district at Napandee and Lyndhurst.
A community ballot in the Kimba district showed 61.6 per cent of residents supported a facility at either site.
However a separate ballot for Barngarla Determination Aboriginal Corporation members returned 83 valid 'no' votes and zero 'yes' votes.
Mr Canavan is yet to make a final decision on whether either Kimba site has broad community support, with business and neighbour surveys still underway in the district.
"I will make a formal announcement early next year on the site selection process, and would like to thank everyone who participated in the ballots and other consultation mechanisms," he said.